Main problem to the shrimp culture failure is disease attack caused by parasites, viruses and bacteria. One of the efforts done for disease control in the shrimps culture is by disease prevention by means of increasing shrimp immune system.
Increasing shrimp immune system against disease could be done through immunostimulant administration (Treves-Brown, 2000), one of them using polysaccharide compound. Exopolysaccharide sulfate is a polysaccharide produced by microalgae and applied as anti virus agent, healthy food, antioxidant, anti inflammation and as part of immunomodulatory system (Raposo et al., 2013).
Microalgae diatomae contains polysaccharides with a wide structural rate and can be explored as an active biological content (Skjermo et al., 2006). Polysaccharides extract from C. mulleri could help enhance immune system of Gadus morhua L. (Skjermo et al., 2006). Polysaccharides extract A. orientalis could help increasing immune response and survival rate of shrimps against infection of White spot syndrome virus (Manilal et al., 2009). Polysaccharides produced by Arthrospira plantesis and Porphyridium purpureum resists anti virus strain activity of V. virus dan E. virus (Radonic et al., 2010).
Chaetoceros sp. diatomae was known to have a high antioxidant activity, more than 90% (Natrah, 2007), and produce Hexadecatrienoic-6,9,12-acid and Hexadecadienoic-9,12-acid (Wang, 1999). Whole cell C. ceratosporum in woof can indirectly increase immune response of tiger shrimp, C. mulleri contains β-D-(1-3)-glucan while C. debilis contains β-D-(1-3,1-6)-glucan. β-glucan is an imunostimulative polysaccharide derivative. How the injection of hot water extract C. ceratosporum (HWEC) influences immune response of L. vannamei infected by IMNV is not yet known. Therefore, the objective of this research is to know immune response change of L. vannamei injected with HWEC against IMNV infection, covering change in THC (total haemocyte count), TPP (total protein plasma), phenoloxidase (PO) activity, respiratory burst (RB) activity and shrimp survival rate (SR).
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(Author: Erika Saraswati, Arief Prajitno, Uun Yanuhar, A. Latief Abadi